Study on denervated rat skeletal muscle by 7.0 T 31P-MR phosphorus spectroscopy / 中华放射学杂志

Objective@#To investigate the changes of 31P-MRS in denervated skeletal muscle at 7.0 T MR system.@*Methods@#In the experiment group, a total of 18 male Sprague-Dawley rats aged 6-8 weeks old and weighing 200-250 g were obtained. The right posterior femoral nerve were transected, and the proximal stumps were ligated by using 5-0 nylon stitches to preclude spontaneous repair. A sham surgery (incision and exploration of the nerve) was performed at the same time (n=6). Before rat model established and at varying times after initial surgery (3 d, and 1, 2, 4, 6, and 8 weeks). 31P-MR spectra of rat quadriceps femoris were acquired on 7.0 T Agilent small animal MR imaging system. We quantified the phosphocreatine (PCr), adenosine triphosphate(β-ATP), and inorganic phosphate (Pi) using Creatine phosphate disodium salt(10 mmol/L) as an external standard. The ratios, include PCr/Pi, Pi/β-ATP, PCr/β-ATP, PCr/(PCr+Pi) and Pi/(PCr+Pi) were quantified in jMRUI. All data were analyzed by one way analysis of variance (ANOVA) with posttest inter-group comparisons using Bonferroni test. Comparative analysis methods between the experimental and control group were performed via independent samples t test. P<0.05 was considered as statistically significance.@*Results@#The average measured concentrations of β-ATP, PCr, and Pi in control groups were (6.654±0.178) μmol/g, (25.656±0.738) μmol/g, and (1.594±0.096) μmol/g, respectively. There were significant statistically differences in β-ATP, PCr, Pi, PCr/Pi, PCr/PCr+Pi,Pi/PCr+Pi between the study and control group(P<0.05) at any measurement time point after denervation. Pi/β-ATP,PCr/β-ATP and pHi in the experimental group significantly statistically differed from those of the control group (all P<0.05)at each time point except on day 3 after operation. The total concentrations of β-ATP and PCr were reduced by 11.5% and 19.7% respectively on day 3. Thereafter, β-ATP and PCr declined rapidly by 63.1% and 68.8% at week 4 respectively, then decreased slowely by 74.0%和82.3% till week 10. The change of PCr/Pi is similar to β-ATP and PCr, but more remarkable. Pi, Pi/PCr+Pi, Pi/ATP showed a progressively increase till week 10. The intra-cellular pH (pHi) of normal rat muscles was 7.033±0.017, While the pHi gently in experiment rat muscles decreased during the entire experiment.@*Conclusion@#31P-MRS with 7.0 T can quantify the temporal changes of energy metabolism and pHi in normal and denervated rat muscles. It shows that the dysfunction of energy metabolism are progressive with time and that they begin within a short period following the nerve section, the change of β-ATP, PCr, PCr/Pi take place primarily within 4 weeks after denervation. β-ATP, PCr, and PCr/Pi may be potential biomarkers of energy metabolism in the evaluation of denervated muscle atrophy.

Safety and short-term efficacy of MR guided focused ultrasound surgery for bone metastasis-induced pain palliation / 中华放射学杂志

Objective To discuss the safety and short-term efficacy of MR-guided focused ultrasound surgery (MRgFUS) for pain palliation of bone metastases patients.Methods Fourteen patients with painful bone metastases were recruited in this prospective study.The treating efficacy was characterized by numerical rating scale (NRS),the brief pain inventory quality of life (BPI-QOL) survey,and Karnosky performance status scale (KPS).Adverse events occurred pre-and post-treatment were analyzed.Normal distributed statistics was analyzed by using paired-samples t test or Wilcoxon rank sum test.Results Fourteen patients were treated with MRgFUS,2 patients dropped out of the study.The NRS ratings are 6.50(4.00),5.00 (5.25),2.50(5.00),2.50(4.75),2.00 (6.00) for pre-treatment,one week,one month,two months,and three months,respectively.Such variances of NRS ratings were statistically significant (Z=-2.773,-2.740,-2.769,-2.675;P＜0.05).The BPI-QOL ratings were (42.42± 8.27),(30.67 ± 12.29),(29.17±15.38),(29.92± 17.67) and (35.67± 19.28),respectively.The BPI-QOL ratings decreased in the first two months after the treatment which is statistically significant (t=3.231,2.820 and 2.453;P＜0.05);whereas for the third month,the BPI-QOL rating was statistically insignificant compared with the one before the treatment (P＞0.05).The KPS ratings were 80(28),80(20),65(45) for pre-treatment,one week and three months after treatment,respectively.Three months after the treatment,the KPS ratings decreased which was statistically significant compared with the one before the treatment (Z=-2.204,P＜0.05).After the treatment,one patient developed deep venous thrombosis,three patients reported lower extremities numbness,two patients had soft tissue edema around the lesions.Conclusions MRgFUS is effective for short-term pain palliation of bone metastases.Such noninvasive technique is safe and can improve patients' living condition.

Application of MR angiography and perfusion weighted imaging in the evaluation of abnormal vessels in Moyamoya disease, cerebral revascularization after operation and cerebral hemodynamics during the perioperative period / 中华放射学杂志

Objective To evaluate the clinical value of MRA on the abnormal vessels in Moyamoya and cerebral revascularization , and to evaluate PWI in the observation of cerebral hemodynamics before and after cerebral revascularization.Methods Twenty-four patients with Moyamoya disease ascertained by DSA received cerebral revascularization on one side.MRA and PWI were performed for all patients before and after the operation , while DSA was performed after operation in nine patients to compare the images of MRA and DSA by three experienced radiologists.Perfusion parameters in terminal branches of middle cerebral artery ( MCA) on the operative side were compared with those on the contralateral sides and the cerebellum , including cerebral blood flow (CBF), cerebral blood volume (CBV), mean transit time (MTT), and delay time ( DT) , as well as relative ratio ( values on the operative side/values on the contralateral side , and values on the operative side/values on the cerebellar region ) of perfusion parameters (rCBF, rCBV, rMTT and rDT) were calculated.Comparisons of the data between different groups were performed using paired Student′s t test.Results MRA was similar to DSA in displaying the internal carotid artery , main branches stenosis, Moyamoya vessels, and cerebral revascularization.DSA was better in displaying collateral vessels than MRA, but primary images of MRA provided anatomic and pathologic information of cerebral parenchyma.After the operations , rCBF (1.30 ±0.27) and rCBV (1.26 ±0.21) of MCA perfusion regions on the operative and the contralateral sides were higher than rCBF (0.73 ±0.15) and rCBV (0.98 ±0.12) before the operation significantly (t=-7.19,-6.64,P<0.05).rMTT (1.06 ±0.20) and rDT (1.07 ± 0.18) after the operation were lower than rMTT(1.53 ±0.34)and rDT (1.40 ±0.26) before the operation (t=5.62,5.40,P<0.05) .In MCA perfusion regions on the operative and cerebellar sides , rCBF(1.93 ± 0.34)and rCBV(2.25 ±0.35)were higher than rCBF(0.88 ±0.18)and rCBV(1.16 ±0.22)(t=-3.04,-3.06,P<0.05) before the operation.rMTT (1.13 ±0.29) and rDT (1.29 ±0.12) were lower than rMTT (1.88 ±0.19 ) and rDT ( 3.29 ±0.47 ) before the operation ( t =4.01, 4.72, P <0.05 ).Conclusions MRA is a safe , reliable method to evaluate abnormal vessels and cerebral revascularization after the operation in moyamoya disease.PWI can detect poor cerebral perfusion , and can be used for assessment of the effect of cerebral revascularization.

MR features of mice spleen lymphocytes labeled with super-paramagnetic iron oxide particles / 中华放射学杂志

Objective To investigate the feasibility of labeling mice spleen lymphocytes with superparamagnetic iron oxide(SPIO)and in vitro MR imaging of the labeled cells.Methods Spleen lymphocytes of 5 mice were isolated and then labeled with SPIO of 100,50,25,15,10,5 μg/ml,which was previously prepared with PLL.Prussian blue staining was performed to show the intracellular iron.Cell viability was compared among fresh,labeled and unlabeled cells.Different concentrations of mice spleen lymphocytes were screened using 3.0 T MR on T2WI,T2 * WI and SWI sequences in vitro.Cell viability was compared using independent-sample t test between groups.The MRI values among different groups were compared using one-way ANOVA.Results SPIO prepared with PLL could successfully label mice spleen lymphocytes,the optimum concentration of SPIO was 5 μg/ml.The Prussian blue staining showed intracellular blue spots and a labeling efficiency of(93.6 ± 2.1)％.Three groups of fresh,labeled and unlabeled cells showed a Trypan blue staining result of(94.8 ± 3.1)％,(88.7 ± 2.7)％,and(88.9 ±3.2)％,respectively; no statistically significant difference was found in cell viability between labeled and unlabeled lymphocytes(t =0.281,P ＞ 0.05); however,the cell viability of fresh cells were statistically significant higher than the labeled and unlabeled lymphocytes(t =8.125 and 7.253 respectively,P ＜0.05for all).Among the T2 WI,T2 * WI and SWI sequences under the same concentrations of cells,the SWI sequence was the most sensitive.Conclusions The mice spleen lymphocytes can be effectively labeled with SPIO with no impact on cell viability,and MR can be used to track these labeled cells in vitro.The SWI sequence is the most sensitive.

Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia / 中华妇产科杂志

Objective To study expression and activation of p38 mitogen activated protein kinase (MAPK) in vascular endothelial cells dysfunction in preeclampsia. Methods From Sept. 2009 to Mar.2010, 54 pregnant women underwent deliveries in the First Affiliated Hospital of Chongqing Medical University were enrolled in this study, including 20 patients in mild preeclampsia group, 16 patients in severe preeclampsia group and 18 women with term cesarean section without perinatal complications as control group. Placental endothelial cells were labeled by CD34 to assay microvessel density (MVD) of each group. Immunohistochemical SP and western blot were used to detect localization and expression of p-p38 MAPK protein, respectively. The levels of sera soluble fms-like tyrosine kinase-1 (sFlt-1)and soluble endoglin(sEng) were measured by ELISA. Results ①The MVD of placenta were 103 ± 3 in control group, 81 ±5 in mild preeclampsia group and 63±4 in severe group, respectively, which showed statistical difference among each group (P＜0.05).②The expression of p38 MAPK protein were 0.84±0.05 in control group,0.90±0.14 in mild group and 0. 86 ±0.18 in severe group, which did not reach remarkable difference among each group (P＞0.05). The expression of p-p38 MAPK protein were 0.13±0.05 in control group,0.59±0.12 in mild group and 1.16±0.18 in severe group, which show statistical difference among each group(P＜0.05).(3) The localization of p-p38 was in trophoblast, endothelial cells and a few (5.2±0.3)and(10.9±0.4)μg/L in control group,(12.5±1.2) and (20.4±5.3)μg/L in mild group and (19.3±3.0) and (29. 5 ±3.7) μg/L in severe group. When drawing paired comparison in those p-p38 MAPK protein levels and the concentrations of serum sFlt-1, sEng in preeclampsia groups (r=0.68,P＜0.05;r=0.87,P＜0.05). Conclusions The remarkable activation of the p38 MAPK in the placenta of patients with preeclampsia induced the increased levels of sFlt-1 and sEng in maternal serum, which confer the injury of vascular endothelial cells that caused the significant decline of MVD in placentas. p38 MAPK signaling might be one of the key pathways in vascular endothelial cell dysfunction in preeclampsia.

CT and MR Imaging Features of Dysembryoplastic Neuroepithelial Tumor / 实用放射学杂志

Objective To analyze CT and MR imaging features of dysembryoplastic neuroepithelial tumor(DNT),so that to improve its diagnostic accuracy.Methods CT and MRI fidnings of DNT in 10 cases proved surgically and pathologically were retrospectively reviewed.Results All lesions were located in the super-tentorial gray matter regions or involved the cererbral certex mainly.The lesions were round,lobular,triangular or irregular pseudocystic masses with definite borders mostly.The lesions were hypointense on T_1WI and hyperintense on T_2WI.On fluid attenuated inversion recovery weighted images,the hyperintense "ring sign" and septa of the lesions were seen.On diffuse-weighted images(DWI),the lesions were hypointense or slight hypointense.On MR spectroscopy,NAA of the lesions slightly decreased.There were no peripheral edema and mass effect with well-demarcated.7 lesions had no enhancement and 3 lesions had slight unhomogeneous enhancement after administration of contrast medium.The lesions in 2 cases on plain CT showed low attenuation and one had calcification inside the lesion.Conclusion DNTs are of typical neuroradiologic features,that may be helpful for the diagnosis of which preoperatively.

Study of relationship of an androgen receptor CAG repeat polymorphism with postmenopausal osteoporosis / 中华老年医学杂志

Objective To study the relationship between a CAG repeat polymorphism of the androgen receptor (AR) gene and postmenopausal osteoporosis (PMO). Methods Genotypes for the AR polymorphisrn were determined by gene scan and DNA sequence methods in a case-control study,including 78 cases of PMO at femoral neck and 73 cases as controls, and 108 cases of PMO at lumbar spine (L2-4) and 60 cases as controls. Bone mineral density for the proximal femur and L2-4 was measured by NORLAND XR-46 dual-energy X-ray absorptiometry. The relationship between the CAG repeat polymorphism and PMO was investigated. Results Eleven different allelic variants,containing 18, 20, 21, 23, 24, 25, 26, 27, 28, 29, and 30 CAG repeats were detected, 16 genotypes were present in the subjects. There were no significant differences in the genotype and allele distributions of (CAG) n polymorphism between PMO group (SS : 25.6 %, SL : 39.7%, LL : 34.6 % ;S:45.5%,L:54.5%) and control group (SS: 23.3%,SL=45.2% ,LL:31.5%;S:45.9%,L:54.1%) at the femoral neck site (all P＞0.05). The risk of PMO at femoral neck in females with the genotypes of SL (0R:0.798,95%CI:0.335～1.797), the LL (0R:0.998,95%CI:0.425～2.341), and the combined SL and LL (OR:0.880, 95% CI: 0.419～1.852) were not significantly increased in comparison with those of females with the SS genotype (all P＞0.05). There were no significant differences in the genotype and allele distributions of (CAG)n polymorphism between PMO group(SS: 18.5%, SL: 49.1%, LL: 32.4%;S:43.1%, L: 56.9%) and control group (SS: 21.7%, SL:45.0% ,LL:33.3% ;S:44.2% ,L:55.8%) at the L2-4 site (P＞0. 05). The risk of PMO at L2-4 in females with the genotypes of SL (OR:1. 276,95%CI:0. 552～2. 950), the LL (OR:1. 137,95%CI:0.468～2.766), and the combined SL and LL (OR: 1. 217,95% CI: 0. 556 ～2. 663 ) were not significantly increased in comparison with those of females with the SS genotype (all P＞0.05). After adjustments for age, postmenopausal period, menopausal age, and body mass index, the logistic regression analyses revealed the (CAG)n polymorphism was not significantly associated with PMO at the femoral neck and L2-4 site (all P＞0.05). Conclusions The CAG repeat polymorphism in the AR gene may not be associated with PMO at the femoral neck and L2-4 site.

Clinical diagnosis of 5 cases of pituitary stalk interruption syndrome / 中华内分泌代谢杂志

Objective To raise the level of clinical diagnosis for the patients with pituitary stalk interruption syndrome (PSIS). Methods Five patients (4 males) with PSIS were retrospectively analyzed with respects to the clinical features, endocrine status and image characteristics. Magnetic resonance imaging (MRI) scan was performed at hypothalamic-pituitary region. Results The clinical manifestations of all patients consisted of growth retardation and delayed puberty without polyuria. Deficiency of multiple anterior pituitary hormones was revealed in all patients by the evaluation of endocrine status. The features of MRI included a lack of visible pituitary stalk, absence of posterior lobe hypersignal in the sella turcica and a hyperintense spot in the region of the thalamus opticus. Conclusion The clinical characteristics of patients with PSIS are growth retardation and delayed puberty. The evaluation of anterior pituitary function is necessary. The detection of an anatomical abnormality around hypothalamic-pituitary region by MRI is important diagnostic evidence.

Chaotic analysis on cardiac systems of normal pregnant rats and fetuses / 生物医学工程学杂志

<p><b>UNLABELLED</b>This investigation was made to explore the chaotic characteristics of cardiac system and differences of autonomic nervous system development of normal pregnant rats and fetuses. The electrocardiograms, chaotic graphics and digital characteristics of heart period signal (HPS) of normal pregnant rats and fetuses by day 21st were evaluated with a computerized HPS extracting and analyzing system.</p><p><b>RESULTS</b>(1) The mean values of frequency and voltage of fetal rats electrocardiograms were lower than those of pregnant rats significantly (P < 0.01). (2) Being similar to those in human, the chaotic graphics showed three-amplitudes spectral features in both normal pregnant rats and fetuses. However, the second amplitude and third amplitude were lower in fetal rats than in pregnant rats. (3) The Heart Relative Dispersion, Lyapunov Exponent and Fractional Dimension were significantly lower in fetal rats than in pregnant rats (P < 0.01). These findings demonstrated that the autonomic nervous system development of fetal rat was still immature and it exhibited lower complexity of HPS and chaotic degree than that of pregnant rat did.</p>

Women's sexual health after delivery and its related influential factors / 中国组织工程研究

BACKGROUND: The postnatal sexual health of women is crucial in reproductive health, due to lack of sexual knowledge and sexual heath care service as well as conventional idea, the medical staff and postnatal women themselves pay little attention and discussion on the sexual health after childbirth.OBJECTIVE; To investigate the prevalence and types of women's sexual problems after childbirth, identify the factors associated with dyspareunia and investigate the status quo of postnatal sexual health care service. DESIGN: Cross-sectional study and follow-up survey within six months after delivery.SETTING: Department of Reproductive Medicine, Chongqing University of Medical Sciences. PARTICIPANTS: A total of 798 primiparous women delivering a live birth in Department of Obstetrics at the First Affiliated Hospital of Chongqing University of Medical Sciences between November 2000 and July 2001, and 79 husbands who accompanied their wives during the follow-ups were adopted in this study.METHODS: The primiparous women after 6 months of childbirth and husbands conducted routine postnatal follow-ups and were informed of the follow-up content. The interview was used in this cross-sectional study and the questionnaires were finished under the help of health professional. And some advice, consultations and treatments can be given in time. MAIN OUTCOME MEASURES; ①main change of postnatal sexual life;②postnatal sexual problems and related factors. RESULTS: Totally 460 out of 798 primiparous women in accordance with the inclusive and exclusive criterions received follow-up surveys after telephone or letter notification. ①Main change of postnatal sexual life: Of the 460 respondents, 94.74% had resumed sexual activity within six months after delivery. Compared with one year pre-pregnancy, the number of sexual activity decreased obviously within 3-6 months after delivery, and 66.0％ of postnatal women decreased the sexual desire.②Postnatal sexual problems and related factors: Sexual morbidity increased significantly after the childbirth: In the first three months after delivery, 70.6％ of women experienced sexual problem(s), declining to 55.6％ during the 4th-6th months, and reduced to 34.2％ at the 6th month, but not reaching pre-pregnancy levels of 7.17％. The sexual problems of women after delivery included dyspareunia, vaginal dryness, orgasm disorder and vaginal relaxation, etc.4. And dyspareunia was the most common type. Between the 4th and 6th month after delivery, dyspareunia was significantly associated with the effect of traditional sexual ideas, experience of dyspareunia before pregnancy and current breastfeeding (P ＜ 0.05), while the association of dyspareunia with delivery mode was not significant.③Sexual care service and sexual counseling after delivery: Only 20.8％ of women received information about sexual health from health care personnel within 42 days after delivery, and consultation rate of sexual intercourse problems was 8.0％.④Husbands' comprehension of postnatal problems in women: Only 59.2％ of partners whose wives had postnatal sexual problem(s) knew that their wives had ever experienced sexual problem(s) and 8.1％ of partners had sought medical help. CONCLUSION; Sexual health problems are very common in women after childbirth, taking up for 70.6％. And the quality of postnatal sexual health is unrelated with delivery mode, but postnatal health care service are often overlooked, additionally lack of professional counseling and treatment. The current practices of encouraging the husband's supports after the childbirth may well influence the prevention and treatment of postnatal sexual problems in women.

Improvement on isolation and culture of human endometrial glandular epithelial cells and stromal cells in vitro / 重庆医科大学学报

Objective:To explore the isolation and culture of a better yield of purified human endometrial glandular epithelial cells and stromal cells. Methods:Digestion with high concentrated collagenase and DNase,filtratinn and adhesion purification were used to isolate,purify human endometrial glandular epithelial cells and stromal cells in 30samples. And the two kinds of cells were reproduced. Results:The endometrial cell culture was successfully established in 29 of 30 endometrium samples. From one gramme of endometrial tissue,the yields of glandular epithelial cells and stromal cells were (40～50)?10~6 and (40～50)?10~6 respectively. The purities of glandular epithelial cells and stromal cells were 96% and 98% respectively. Stromal cells could be reproduced into several passage. However,glandular epithelial cells could not be reproduced. Conclusion:A better yield of purified human endometrial glandular epithelial cells and stromal cells can be obtained by the modified culture procedure.

Change of heart rate power spectrum and its association with sudden death in the fetuses of rats with intrahepatic cholestasis of pregnancy / 生物医学工程学杂志

<p><b>UNLABELLED</b>To investigate the relationship between imbalance of cardiac autonomic nervous system and sudden death in fetuses of rats with intrahepatic cholestasis of pregnancy (ICP), the animal model of ICP was induced by hypodermic injection of 17-alpha-ethinylestradiol and progesterone. The electrocardiograms and frequency domain analysis of heart rate variability (HRV) including low frequency (LF), high frequency (HF) and the ratio between low and high frequencies (LF/HF) of fetal rats by the 21st day of gestation were evaluated with Chart 5 software of Powerlab biologic signal extracting and analyzing system.</p><p><b>RESULTS</b>(1) The serum total bile acids (TBA) levels of pregnant rats were (78.5 +/- 4.5) micromol/L in Group ICP and (24.6 +/- 3.6) micromol/L in Group control; significant difference was noted between the two groups (P < 0.01). (2) In Group ICP, fetal rats arrhythmias appeared after (29.3 +/- 6.4) minutes' observation, and fetal rats died suddenly after (23.5 +/- 4.6) minutes' arrhythmias; However, the fetal rats in Group control all showed normal electrocardiograms over 90 minutes' continuous observation. (3) The values of LF and LF/HF of fetal rats in Group ICP within 20 minutes before fetal rats arrhythmias were significantly higher than those in Group control (LF 48.45 +/- 4.11 nu vs. 33.87 +/- 4.31 nu, and LF/HF 0.99 +/- 0.14 vs. 0.61 +/- 0.10, respectively, P < 0.01). (4) Dynamic power spectral analysis of HRV indicated that the values of LF and LF/HF of fetal rats in Group ICP increased progressively within 15 minutes before the sudden death of fetal rats. These demonstrated that autonomic imbalance in association with increased sympathetic activity has been strongly implicated in the pathophysiology of fetal arrhythmogenesis and sudden death in ICP. HRV analysis could be a useful tool for fetal surveillance, especially for ICP.</p>

Suggestions for Obstetrics and Gynaecology Teaching with Multimedia / 中华医学教育探索杂志

In order to perfect teaching method,strengthen the teaching effect,we apply the multimedia teaching mold.We will give some positive suggestions for the optimization of the field and track teaching process for improving teaching quality.The multi-media aided-teaching should be better utilized for the requirements of teaching and learning to improve the teaching quantity.

Effect of imitated CO_2 pneumoperitoneum on expressions of Survivin and Bcl-2 in human cervical carcinoma cell line / 第三军医大学学报

Objective To study the effect of CO2 pneumoperitoneum on the anti-apoptotic capability of human cervical carcinoma cell line(CaSki).Methods The imitated CO2 pneumoperitoneum was established in vitro.After CaSki cells were exposed to CO2 pneumoperitoneum at 0,7 or 14 mmHg for 1,2,3,4 h,the expressions of Survivin mRNA and Bcl-2 protein were examined by RT-PCR and Western blot respectively.The untreated CaSki cells served as control.Results Under 7 or 14 mmHg,the expressions of Survivn and Bcl-2 had an increasing tendency with the exposure time prolongation.Within the same exposure time,the expressions of Survivin and Bcl-2 under 7 mmHg were significantly higher than those under 0 or 14 mmHg or in control group.Conclusion Under certain pressure and within certain exposure time,CO2 pneumoperitoneum could improve anti-apoptotic capability of human cervical carcinoma cells,which may be one reason for the promoting effect of carbon dioxide pneumoperitoneum on malignant cells growth.

Effects of carbon dioxide pneumoperitoneum on the invasive ability of ovarian carcinoma cell line SKOV3 / 第三军医大学学报

Objective To investigate the effects and mechanism of carbon dioxide (CO_ 2 ) pneumoperitoneum on the adhesive, migratory and invasive abilities of ovarian carcinoma cell line SKOV_ 3 . Methods SKOV_ 3 cells were exposed to an CO_ 2 insufflation medium. The cells′ adhesive ability to Matrigel was analyzed by MTT assayand, and their migratory and invasive abilities were analyzed through Boyden filters metastasis model. CD44 and bFGF expressions were examined by immunohistochemistry and semi-quantitative RT-PCR. Results Cells exposed to an in vitro pneumoperitoneum demonstrate could significantly increase adhesive, migratory and invasive abilities compared to the control group (P

Expression of survivin in endometriosis and its relationship with bcl-2 and bax protein / 第三军医大学学报

Objective To study the relationship of apoptosis-related gene survivin with bcl-2, bax protein in endometriosis. Methods The expressions of survivin, bcl-2 and bax protein were detected by immunohistochemical method (SP) in ectopic endometrium from 40 cases, and compared with that in eutopic endometrium from 25 cases without endometriosis. Results The expression rate of survivin protein in endometriotic cells was much higher than that in normal endometrial cells. The survivin was not correlated with the phase of menstrual cycle , but positively correlated to bcl-2, negatively to bax. Conclusion Survivin may play an important role in pathogenesis of endometriosis. With survivin gene, apoptosis-related gene bcl-2 may have a synergic role and bax have an antagonistic role in the formation and progression of endometriosis.

Expression of VEGF and MMP9 in human ovarian carcinoma cell line SKOV_3 after carbon dioxide treatment / 第三军医大学学报

Objective To study the effects of carbon dioxide on the metastatic capability of ovarian carcinoma cell line. Methods After ovarian carcinoma cells SKOV_(3) were treated with 100% carbon dioxide for 2 h, the expressions of VEGF and MMP9 were examined by immunohistochemistry and RT-PCR. Results After the treatment of carbon dioxide for 2 h, the expression of VEGF and MMP9 was significantly increased at 12~(th) h, the expression of VEGF reached the peak at 24~(th) h and the expression of MMP9 reached the peak at 48~(th) h. Conclusion Carbon dioxide may improve the metastatic capability of ovarian carcinoma cell line.

Clinical application of 3D reconstruction of tracheobronchial tree with electron beam CT / 中华放射学杂志

Objective To explore the clinical promise of CT 3D reconstruction of tracheobronchial tree(TBT) by analyzing 73 cases retrospectively. Methods All the 73 cases were collected from October 1997 to February 2000, who were scanned by EBCT with 130 kV and 630 mA. The scanning method was continuous volume scan, the slice thickness were 3 mm or 1.5 mm. All cross-sectional images were transmitted to the INSIGHT workstation and reconstructed with SSD (shaded surface display), and the threshold setting were -500 to -300 HU. Results 3D reconstruction of TBT with EBCT could reveal the abnormal changes of TBT by many kinds of diseases including central cancer, inflammation, bronchiectasis, saber-sheath trachea, trachea cancer, congenital disorders, post-surgical changes of lung cancer, and stenoses by adjacent benign or malignant diseases. It could be used to locate the stenoses and measure stenostic extent. Of the 35 central cancer cases with 3D reconstruction, 6 cases were pestle obstructed, 15 cases cone obstructed, 5 cases interrupted irregularly, 8 cases with eccentric stenoses, and 1 case with right stem destroyed and right upper lobe bronchus obstructed. Conclusion 3D reconstruction of TBT has characteristic sign in the diagnosis or differential diagnosis of central airway′s benign or malignant stenoses, and it is of instructional value in clinical use.

Effects of nitric oxide synthase inhibitors on cerebral mitochondrial structure and function in fetal rats with intrauterine distress / 中华妇产科杂志

Objective lo evaluate the effects ot different types oi nitric oxide synthase inhibitors on cerebral mitochondrial structure and function in fetal rats with intrauterine distress. Methods Rats were divided into control group, acute ischemia group, treatment group 1 injection of [ N omega-nitro-L-arginine (L-NNA) 4 mg/kg into pregnant rats' abdomen before ischemia], reperfusion group, treatment group 2 [injection of L-NNA 4 mg/kg into pregnant rats' abdomen before ischemia followed by injection of aminoguanidine (AG) 500 mg/kg before operation]. Changes of mitochondrial structure were observed by transmission electron microscopy and expression of neuronal nitric oxide synthase (nNOS) mRNA (A) through RT-PCR. Inducible nitric oxide synthase ( iNOS) activity and mitochondrial Na+ K + -ATPase and cytochrome oxidase activity were measured. Results (1) The A of NOS(5 min,15 min) in acute ischemia group was higher than that of treatment group 1 ( P 0. 05). But the A of nNOS in two groups was higher than that in control group ( P 0. 05 ). Mitochondrial Rsv in reperfusion group was all smaller than those in treatment group 2. And mitochondrial Rsv in all the groups was smaller than that in control group( P

MR Imaging Diagnosis of the tumors in the Region of Jugular Foramen / 实用放射学杂志

Objective To study the manifestations of MR of the tumor in jugular foramen area(JFA) and its diagnostic values.Methods 13 cases of tumors confirmed by pathology in JFA were reported,and the characteristics of MR of different kinds of tumor were analysed.Results There were its own MRI manifestations of different tumors in JFA,and the localizing and qualitative diagnosis of these tumors can be accurately made by MRI.Conclusion MRI is of very important diagnostic values in detecting the tumors in JFA.